Characters
Original Characters
- Leonardo a.k.a. Leo (レオナルドa.k.a. レオ, Reonarudo a.k.a. Reo); the leader.
- Raphael a.k.a. Raph (ラファエルa.k.a. ラッフ, Rafaeru a.k.a. Raffu); the brawn and he becomes the Night Watcher later on.
- Donatello a.k.a. Donnie or Donny (ドナテッロa.k.a. ドニー, Donaterro a.k.a. Donī); the brains.
- Michelangelo a.k.a. Mikey (ミケランジェロa.k.a. マイキー, Mikeranjero a.k.a. Maikī); the funny one.
- Splinter (スプリンター, Supurintā); the rat of Master Yoshi and the turtles sensei. Splinter knows how his turtles feel about each other.
- Mei Pieh Chi a.k.a. Venus de Milo (美培智 a.k.a. ミロ• (金星)•の•ヴィーナス, a.k.a. Miro (Kinboshi) no Vuinasu, lit. "Beautiful Turtle of Energy"); the mutant female turtle from Ninja Turtles: the Next Mutation, TV series. She is of no relation to the boy turtles that until now! Venus is now the male turtles long lost sister.
- Mona Lisa a.k.a. Mona (モナ•リザ a.k.a. モナ, Mona Riza a.k.a. Mona); She was a college girl who was captured by a captain named Filch and when she tried to sabotage (or destroy) the submarine; she got turned into a mutant lizard. She made her appearance in the episode of the 1987 TMNT TV show "Raphael Meets His Match" episode and was she really Raph's (former) girlfriend?
- Cody (コーディ, Kodi); the human male descendant of April and Casey from Fast Forward!
- Serling, the robot (サーリング、ロボット, Sāringu, robotto); Cody's robot babysitter?
- April O'Neil (エイプリル•オニール, Eipuriru O'Nīru); the news reporter and she give the news from top side.
- Arnold Bernid "Casey" Jones (アーノルド•ベラニド• "ケーシー" •ジョーンズ, Ānorudo Beranido "Kēshī" Jōnzu); the vigilante.
- Oroku Nagi (オロク•ナギ); is the older brother of Oroku Saki and in love with Tang. He was killed by Yoshi.
- Shredder a.k.a. Oroku Saki (シュレッダーa.k.a. オロク•サキ, Shureddā); is the younger brother of Oroku Nagi.
- Karai (唐井); Shredder's daughter.
- Yoshi Hamato (ヨシ•ハマト); He once became Splinter in one universe, otherwise he's dead to all others (or all other universes).
- Tang Shen (汤奢嗯); She loved Yoshi and is now dead.
- Niwa (丹羽/ 庭/にわ, lit. "Garden"); Yoshi's daughter.
- Chung (チョン, lit. " intelligent"); Venus's mentor and adopted father.
- the Foot Clan (フットクラン, Futto Kuran); the dark clan?
- Tengu (天狗, lit. " Braggart")
New Characters
- Lucia a.k.a. Lu (ルチアa.k.a. ル, Ruchia a.k.a. Ru); the leader in blue and Leo's soul mate. / Element: Ice & Invisible / Weapon: Samurai's Sword*(name pronounced: Lu.cee.ah)
- Rossi a.k.a. Ros (ロッシa.k.a. ロス, Rosshi a.k.a. Rosu); the hothead in pink and Raph's soul mate. / Element: Flame / Weapon: Kunai Ring Daggers (lots)(name pronounced: Rosé or Rosy)
- Diana a.k.a. Dia (ダイアナa.k.a.ディア, Daiana a.k.a. Dia); the brains in violet and Donnie's soul mate. / Element: Thunder / Weapon: Shakujou (Shank or Staff)(name pronounced: d.ah)
- Marietta a.k.a. Mari (マリエッタa.k.a. マリ, Marietta a.k.a. Mari) ; the jokester in yellow and Mikey's soul mate. / Element: Wind / Weapon: Batons (name pronounced: mary.etta or Marie)
- Sakura (サクラ); the rat, sensei of the turtles and named after Nobi's late wife.
- Sensei Nobi Miyazato (先生•野比•のび•宮里); the turtles first sensei. He disappears in a struggle.
- Sakura Miyazato (さくら•宮里); Nobi's wife and Seijii's mother.
- Seiji Miyazato (セイジ•宮里); Nobi's son, the turtles` brother and friend.
- Lucca Laird (ルッカ•レアード, Rukka Reādo); She is a new ally to the TMNT and TMKT. She was born and raised in America and she's a runaway rebel. She loves looking at graffiti even though she's never gets involved with the crime of defacing property. She wants to go to Japan someday and she learns as much of the Japanese language as she can.
- Ethan Laird (イーサン•レアード, Īsan•Reādo); Lucca's older brother, the rebel.
- Marlene Laird (マレーネ•レアード, Marēne•Reādo); Lucca's younger sister, the cute one.
- Hana (ハナ, lit. "flower"); the girl who rescued the baby turtles from harm.
- Zhong de You (GT) a.k.a. Mars de Milo (忠的友a.k.a. マルス•(火星)•の•マイラ, Zhōng de You a.k.a Mazu (Kasei) no Maira (de Mira), lit. "Myra of Mars" or "Loyal Friend"); the mutant male turtle from TMNT meets TMKT series. he might have been of no relation to the girl turtles, but now he is the female turtles long lost brother and Venus de Milo's mate.
- Tao Yi (涛义[涛义], lit. "Great Waves that is firm and resolute," "Great Waves of Justice"); Yi is Zhong's sensei and adopted human father.
- Tao Chao (超, lit. "Surpass"); Chao is Yi's younger brother.
- Leon Raps (レオン•ラップ, Reon Rappu); Mona Lisa's new soul mate.
- Fang a.k.a. Yan Fe (ファングa.k.a. ヤウン•フェイ, Fangu a.k.a. Yaun Fei, lit. " fragrant " or " swallow bird; gorgeous"); the Claw Clan's leader and the female turtles enemy. Does she have the secret element of the "Phoenix" locked up inside her?
- Kuroto (黒ト, lit. " Black port"; a samurai villain met later on...
- Jimone (ジモネ); the owner of China Town "Clandestine" restrant and old friend of Nobi, but s new friend of the turtles. (name pronounced: Jim.moan)
- Murasaki (ムラサキ, lit. "Purple"); who this person is, is still unknown.
- Momoiro (ももいろ, lit. "Pink"); ; who this person is, is still unknown.
- Ming-huá (, , lit. "tomorrow's flower"); who this person is, is still unknown.
- the Storm Clan (嵐の一族, Arashi no ichizoku, lit. "Clan of Storm"); is an air or wind tribe.
- the Claw Clan (鉤爪一族, Kagidzume no ichizoku, lit. "Clan of the Claw"); later changed from the "Storm Clan" to "Claw" becomes earth related.
- Monkey Turtle Ninja (猿忍者亀[モンキー忍者タートル], Saru ninja kame[Monkī ninja tātoru]) ; who this person is, is still unknown.
*One Long Katana, instead of two